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. 2009 May 11;77(7):2691–2702. doi: 10.1128/IAI.01570-08

FIG. 2.

FIG. 2.

Streptomycin treatment alters the microbial composition of both the ileum and the cecum. (A) Rarefaction analysis and 95% confidence intervals are shown for the 16S rRNA-encoding sequences obtained from each site in the untreated and streptomycin-treated mice. Central lines represent rarefaction analysis of each site with the surrounding lines representing the upper and lower 95% confidence intervals. For rarefaction analysis, an OTU was defined as 97% sequence similarity. (B) 16S rRNA gene clone libraries were created from samples containing ileal tissue and contents from untreated mice and those pretreated with streptomycin. Sequences were assigned to OTUs using a definition of 97% sequence similarity. A heat map is used to show the relative abundance of OTUs, with specific OTUs detected in the sample oriented along the horizontal axis, and the dendrogram showing the distribution of OTUs. Darker coloring within the heat map indicates greater representation of specific OTUs. Phyla are shown above the figure, and highly represented OTUs are shown below. Untreated mice shown in this figure are the same as those shown in Fig. 1C.