TABLE 5.

Comparison of kva gene transcript levels in the wild-type and regulatory kva mutant strains

Mutant class and gene	Fold differencea
	VA02 (cbl::Tn10)	VA14 (moaR::Tn10)	VA15 (kva15::Tn10)	VA12 (kvgA::Tn10)	VA18 (kvgS::Tn10)	VA19 (kva19::Tn10)	VA25 (csgD::Tn10)
I
mrkC	−	0.320	0.280	0.353	0.429	0.418	0.305
kva7	0.274	0.419	0.336	0.784	0.593	0.556	0.477
kvgA	0.553	1.112	0.537	−	0.612	1.005	0.537
moaR	0.912	−	0.812	0.717	0.898	0.888	0.832
kva15	0.372	0.673	−	0.721	0.461	0.829	0.494
kvgS	0.776	0.595	0.759	−	−	0.862	0.922
kva21	1.059	0.727	0.316	1.027	0.433	0.556	0.658
kva28	0.758	1.099	0.269	0.313	0.346	1.330	0.582
II
galK	0.362	−	−	0.394	0.500	0.894	0.596
cblb	−	−	0.850	−	0.900	0.950	0.588
kva8c	0.092	0.330	0.151	0.281	0.265	0.254	0.178
kva10	0.371	0.311	0.374	0.791	0.566	0.861	0.417
kva19	0.579	0.913	0.552	1.120	0.749	−	0.710
kva20	0.434	0.678	0.455	0.836	0.559	0.615	0.531
kva25	0.951	1.410	1.069	2.347	1.326	1.292	−
galT	0.406	−	0.508	0.374	0.428	0.706	0.642
III
kva9b	1.789	−	2.596	1.982	2.579	4.561	2.667
kva11	0.381	0.470	0.483	0.936	0.597	0.712	0.534
kva13	0.522	0.119	0.493	0.903	−	1.194	0.731
kva17	−	−	0.333	0.743	0.857	0.724	0.819
fimC	0.989	0.108	0.742	1.688	1.161	1.581	1.097
pagO	0.628	1.377	1.014	1.214	0.930	1.195	0.977
ahpC	0.910	1.181	0.777	1.108	0.861	0.976	0.964

^aSemiquantitative RT-PCR analysis was used to determine the relative transcript abundance of every kva gene in wild-type K. pneumoniae and each of the regulatory kva mutant strains. The relative transcript abundance of a particular kva gene in a bacterial strain was measured by quantification of the band intensity of the specific RT-PCR product, which was normalized to that of GAPDH. The level of each kva transcript from the wild-type strain was normalized to a value of 1. The transcripts of kva04, kva22, kva24, kva26, and kva29, which were not detected in wild-type K. pneumoniae grown under the in vitro conditions tested, are not shown. The differences in kva transcript levels between each of the regulatory mutant strains [VA02 (cbl::Tn10), VA12 (evgA::Tn10), VA14 (moaR::Tn10), VA15 (kva15::Tn10), VA18 (evgS::Tn10), VA19 (kva19::Tn10), and VA25 (csgD::Tn10)] are indicated (means of results from two independent determinations). The expression levels of specific kva genes which were downregulated in a particular regulatory kva mutant more than twofold are indicated in bold. “−” indicates that the transcripts of a specific kva gene were not detected under the in vitro conditions tested by two independent experiments.

^bThe transcripts of cbl and kva9 were detected only in the stationary-phase LB cultures.

^cThe kva08 transcripts were detected under the cultivation with M9-glucose medium.