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. 2009 May 18;77(7):2612–2623. doi: 10.1128/IAI.00280-09

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FIG. 4. — ΔprsA2::erm strains have decreased LLO and PC-PLC activity. (A) The hemolytic activity of culture supernatants derived from WT and prfA(L140F) strains in the presence and absence of prsA1 or prsA2 and its complement was determined based on 50% lysis of sheep RBCs. The average values of four independent experiments are shown. Statistically significant differences are indicated as determined by one-way analysis of variance with Tukey's multiple comparison test (*, P < 0.01; **, P < 0.001; ***, P < 0.0001) (GraphPad V.5.0). (B) Detection of PC-PLC-related phospholipase activity of the ΔprsA1 or ΔprsA2::erm mutant and its complement in the presence of the WT (top panel) and mutationally activated prfA [prfA(L140F)] (bottom panel). prfA(L140F), prfA(L140F) ΔprsA2::erm, prfA(L140F) ΔprsA2::erm + pPL2-prsA2, and prfA(L140F) ΔprsA1 strains incubated on egg yolk agar plates exhibited zones of opacity indicative of PC-PLC activity. A representative image from 1 of 10 plates is shown.