TABLE 1.

Bacterial strains and plasmids used in this work

Strain or plasmid	Genotype	Designation	Source or reference
Strains
TOP10	E. coli host strain used for recombinant pPL2 plasmids
DH5α	E. coli host strain for recombinant pKSV7 plasmids
SM10	E. coli host strain for conjugation of pPL2 plasmids
NF-E1254	E. coli BL21 Star (DE3) with pNF-E1251 for PrsA2 protein expression and purification		This work
NF-L100	L. monocytogenes 10403S parent strain		2
NF-L476	L. monocytogenes 10403S actA-gus-plcB		59
NF-L1167	10403S actA-gus-neo prfA(L140F)	L140F	45
DP-L2161	10403S containing in-frame deletion of hly gene	Δhly	27
NF-L1456	NF-L476 with group II intron (targetron) insertion into lmo2219 (prsA2) at position 457	prsA2::T	This work
NF-L1475	NF-L1456 with integrated pPL2-prsA2.	prsA2::T + prsA2	This work
NF-L1587	NF-L1456 with prsA2::T replaced with a ΔprsA2::erm mutation		This work
NF-L1635	DP-L2161 (Δhly) with ΔprsA2::erm	ΔprsA2::erm Δhly	This work
NF-L1637	NF-L1167 [prfA(L140F) with ΔprsA2::erm]	prfA(L140F) ΔprsA2::erm	This work
NF-L1659	NF-L1637 [prfA(L140F) ΔprsA2::erm] with integrated pPL2-prsA2 (pNF1255)	prfA(L140F) ΔprsA2::erm + pPL2-prsA2	This work
NF-L1651	10403S with ΔprsA2::erm	ΔprsA2::erm	This work
NF-L1656	NF-L1651 (ΔprsA2::erm) with integrated pPL2-prsA2 (pNF1255)	ΔprsA2::erm + pPL2-prsA2	This work
HEL 402	10403S with prsA1 in-frame deletion	ΔprsA1	This work
Plasmids
pNF1256	pKSV7 with ΔprsA2 SOE fragment with internal SalI restriction site		This work
pNF1264	pNF1256 with erm resistance cassette inserted at SalI restriction site		This work
pHY304	Plasmid pHY304 containing constitutive erm cassette		26
pNF1251	pET-TOPO N-His with prsA2 open reading frame		This work
pNF1255	pPL2-prsA2 containing prsA2 with 636 bp upstream of start ATG and 68 bp downstream of stop TAA		This work
pNF1520	pNL9146 with targeted targetron group II intron for gene disruption of prsA2 at nucleotide position 457		This work