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. 2000 Sep 12;97(20):10701–10705. doi: 10.1073/pnas.170297297

Figure 1.

Figure 1

Dissociation kinetics of mutant scFv at 25°C. (A) Yeast displaying mutant scFv isolated from a random library was assayed for antigen dissociation rate. The 4-4-20 and VHS95A values were calculated from fluorescence quenching data with soluble scFvs. Dissociation rates for barnase/barstar (45), streptavidin/biotin (46), avidin/biotin (46), and trypsin/bovine pancreatic trypsin inhibitor (BPTI) (47) are indicated for comparison. (B) Dissociation kinetics of purified, soluble 4 M5.3 and 4-4-20 scFvs were assayed by fluorescence quenching. Triplicate traces for 4-4-20 scFv in PBS (150 mM NaCl/10 mM sodium phosphate buffer, pH 8) are shown, and 12 independent replicates are shown for 4 M5.3 in PBS or LSB. τ1/2, half-time for dissociation.