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Binding and dissociation rate constants for soluble scFvs
| scFv | Buffer | kass (× 10−7 M−1·s−1) | kdiss (× 106 s−1) |
Kd, fM
|
|
|---|---|---|---|---|---|
| kdiss/kass | Direct titration | ||||
| 4-4-20 | PBS | 8.0 ± 3.5 | 25,100 ± 500 | 310,000 | 700,000 ± 300,000 |
| LSB | 41.0 ± 8.0 | 4,270 ± 100 | 10,000 | ND | |
| 4 M5.3 | PBS | 0.6 ± 0.03 | 1.6 ± 0.3 | 270 | ND |
| LSB | 2.9 ± 0.9 | 1.4 ± 0.4 | 48 | ND | |
ND, not determined.
