FIG. 3.

Effect of AHR2 knockdown on CYP1A, AHRRa, and AHRRb expression in response to TCDD exposure. AHR2 translation was blocked by an AHR2 MO injected at the two- to four-cell stage in zebrafish embryos. Real-time RT-PCR was used to quantify transcript abundance in whole zebrafish embryos at 48 hpf after exposure to 0.1% DMSO (carrier) or 2nM TCDD. Standard curves derived from plasmid dilutions were used to calculate transcript abundance. Relative fold induction was determined by comparing transcript abundance for each sample to the noninjected (No MO) DMSO control. Error bars represent one standard deviation; n = 3 replicates of 20 pooled embryos. Statistically significant difference in transcript expression compared to Ctrl MO is represented by asterisks (**p < 0.01, ***p < 0.001). Results presented are from one experiment, but data are representative of two separate experiments.