FIG. 4.

Enhancement of TCDD-induced expression of endogenous CYP1A by dual AHRRa/AHRRb MO knockdown in ZF-L cells. ZF-L cells were cotreated with Endo-Porter (MO transfection agent) and MOs targeting AHRRa and AHRRb for 48 h and then exposed to 0.1% DMSO or various concentrations of TCDD for 24 h. Real-time RT-PCR was used to quantify CYP1 transcript expression. Standard curves derived from plasmid dilutions were used to calculate transcript abundance. Relative fold induction was determined by comparing transcript abundance for each sample to the Ctrl MO DMSO. Error bars represent one standard deviation; n = 3 replicates of 6 pooled wells of cells from a 48-well plate. Statistically significant difference in CYP1 induction compared to DMSO for each individual MO treatment is represented by (+) sign (p < 0.05). Statistically significant difference in CYP1 induction between Ctrl MO and AHRRa MO1/AHRRb MO is represented by asterisks (**p < 0.01, ***p < 0.001).