FIG. 6.

Sox9b expression after AHRR knockdown or TCDD exposure. AHRRa or AHRRb translation was blocked by MO injection at the two- to four-cell stage in zebrafish embryos. Real-time RT-PCR was used to quantify Sox9b expression in whole zebrafish embryos at 72 hpf after exposure to 0.1% DMSO (carrier) or 2nM TCDD. Relative fold change was calculated by the 2^−ΔΔCt method by comparison to the Ctrl MO DMSO treatment. Error bars represent one standard deviation; n = 3 replicates of 20 pooled embryos. Statistically significant difference in transcript expression compared to Ctrl MO DMSO is represented by asterisks (*p < 0.05, **p < 0.01, ***p < 0.001).