FIG. 7.

Recapitulation of TCDD-like developmental phenotypes by AHRRa knockdown in zebrafish embryos. AHRRa or AHRRb translation was blocked by MO injection at the two- to four-cell stage in zebrafish embryos. Common TCDD-induced developmental phenotypes, such as pericardial edema, craniofacial malformations, and cardiac deformities, were phenocopied by AHRRa knockdown in DMSO-treated embryos. Phenotype occurrence was confirmed by microinjection of two separate AHRRa MOs. In contrast, embryos microinjected with AHRRb MO did not display any abnormal developmental phenotypes in DMSO-treated embryos. Results presented are representative of at least three separate experiments.