FIG. 8.

PSS in embryos subjected to AHRR knockdown and exposed to DMSO or TCDD. AHRRa or AHRRb translation was blocked by MO injection at the two- to four-cell stage in zebrafish embryos. Image analysis was used to quantify PSS in 72 hpf embryos after exposure to 0.1% DMSO (carrier) or various concentrations of TCDD. PSS was normalized to the average sac size of noninjected, DMSO-treated embryos and expressed as a ratio of individual/mean of control. Error bars represent one standard deviation; n = 10 individual embryos. A two-way ANOVA was performed to determine the significance of combined TCDD exposure and MO knockdown on the severity of pericardial edema measured by changes in PSS. Statistically significant differences in relative PSS compared to Ctrl MO at each TCDD concentration are represented by asterisks (*p < 0.05, **p < 0.01, ***p < 0.001). Results presented are representative of two separate experiments.