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. 2009 May 20;83(15):7706–7717. doi: 10.1128/JVI.00683-09

FIG. 4.

FIG. 4.

High-dose treatment with GSK501015 inhibits HIV-1 integrant formation and elevates levels of episomal HIV-1 cDNA in vivo. The impact of INI treatment (10 mg/kg per day; the experimental design is shown in Fig. 3A) on HIV-1 DNA species abundance was assessed by determining, by real-time PCR, the load of HIV-1 integrants (A) or episomal two-LTR circles (B) relative to a rat GAPDH standard in DNA extracts from splenocytes. The results given for individual animals (left) are arithmetic means plus the standard error of the mean (SEM). The results given for animal groups (right) are geometric means + SEM. Nonparametric statistical analyses were performed by using the Mann-Whitney U test: integrants, 66-fold reduction (i.e., 98.5% reduction; P = 0.026); two-LTR circles, 7-fold increase (P = 0.03), n.a., not analyzed; ↓, no signal detected. *, statistically significant.