FIG. 4.
Infection, cell killing, and progeny virus production in MHVsoR-EGF inoculated human glioblastoma cell lines. (A) Immunoperoxidase staining of EGFR-expressing human glioblastoma U373MG, U251MG, Gi6MG, U87MG, and U87ΔEGFR cells inoculated with recombinant virus MHVsoR-h-His or MHVsoR-EGF using anti-MHV serum K135. (B) Cell viability of glioblastoma U373MG, U251MG, Gi6MG, U87MG, and U87ΔEGFR cell lines after inoculation with MHVsoR-h-His and MHVsoR-EGF. The cell viability was measured at different time points after inoculation and is depicted relative to uninfected control cells. (C) Growth kinetics of MHVsoR-h-His and MHVsoR-EGF in U87ΔEGFR cells. The production of progeny virus in the culture media at different times postinfection was determined by endpoint dilution on murine LR7 cells, and the TCID50 values were calculated.