FIG. 4.
Identification of a common influenza virus NP CD8 T-cell epitope in pigtail macaques. Macaques responding to the pool of NP peptides had T-cell responses to individual or overlapping influenza virus NP peptides assessed on serial fresh blood samples. (A) Macaque 2374 had a robust CD8 T-cell response to NP, as evidenced by IFN-γ and TNF-α expression. The majority of NP-responding CD8 T cells were directed to two overlapping peptides, peptides 80 and 81 (NP401-423) of the pool of 98 overlapping NP 17-mer peptides. Three other macaques responding to the whole NP pool and also responding to these two NP peptides are also shown (lower fluorescence-activated cell sorter plots). (B) Fine mapping of the common NP CD8 T-cell response was performed by ICS on a series of 9- to 10-mer peptides spanning the overlap between NP peptides 80 and 81.