TABLE 1.

Comparison of kinetic and equilibrium constants for mature PR_H69E with those for wild-type PR

Proteina	Kd (μM)	Km (μM)	kcat (min−1)
PRH69E	0.03b	25 ± 5	122 ± 6b
PR (wt)	<0.01c	48 ± 3d	173 ± 3d

^aMeasurements with PR_H69E were made in 50 mM sodium acetate buffer, pH 5, containing 250 mM NaCl at 28°C (this study). wt, wild type.

^bThis is an apparent value based on total protein concentration; active-site titration indicated that only ∼50% of the protein was catalytically competent.

^cSee reference 12.

^dAt 25°C (6).