FIG. 3.

Comparison of the immunogenicity of the HPV-16 L1 capsomeres in C57BL/6 mice. C57BL/6 mice (five mice per group) were injected twice at a biweekly interval with 5 μg of one of the different L1 capsomere constructs. (A and B) One week after the last immunization, blood samples were collected, and sera were analyzed for L1-specific antibody titers by VLP capture ELISA (A) and for neutralization activity with HPV-16 pseudovirons (B). (C) Splenocytes were isolated and examined by IFN-γ ELISPOT analysis to determine the induction of L1-specific cytotoxic T lymphocytes. neg control, negative control.