FIG. 4.

Comparison of the immunogenicity of HPV-16 L1 capsomeres in TLR4^−/− mice. Sv129 × C57BL/6 mice (four mice per group) were immunized three times at biweekly intervals with 5 μg of L1ΔN10, L1ΔN10dCys, or L1ΔN10Δ408-431 capsomeres either with or without the addition of 5,000 EU of LPS. Two weeks after the last injection, blood samples were collected, and L1-specific antibody titers in the sera were determined by VLP capture ELISA. The value for each individual mouse serum sample (•) and the median of each group (horizontal bar) are shown. neg control, negative control.