TABLE 1.
L1 capsomere construct | L1 protein yield (mg)/liter of bacterial culture | Amt (EU/mg) of LPS/mg of L1 |
---|---|---|
L1ΔN10 | 0.27 | 1,750 |
L1ΔN10dCys | 0.23 | 2,400 |
L1ΔN10Δ414-431 | 0.34 | 2,900 |
L1ΔN10Δ408-431 | 0.41 | 850 |
L1ΔN10ΔC29 | 0.50 | 860 |
L1ΔN10dCysΔC29 | 0.45 | 780 |
L1ΔN10Δ414-431ΔC29 | 0.59 | 1,450 |
L1ΔN10Δ408-431ΔC29 | 0.75 | 410 |
The L1 proteins were expressed in and purified from E. coli as described previously by Chen et al. (11). Subsequently, gel filtration chromatography was performed using a Superdex 200 column. Protein amounts were determined by Bradford assay and by densitometry on a Coomassie blue-stained SDS-PAG using the ImageJ software (NIH). LPS contamination was measured using the colorimetric assay QCL-1000 (Lonza). The values in the table are the median values for at least three independent purifications.