TABLE 1.

List of probes and primers for real-time quantitative reverse transcription-PCR for detection of chicken cytokine mRNAs

Target RNA	Probe or primer sequence (5′→3′)a	Exon boundary	Accession no.	Reference
28S	Probe: 5′-(FAM)-AGGACCGCTACGGACCTCCACCA-(BHQ)-3′		X59733	38
	Forward primer: 5′-GGCGAAGCCAGAGGAAACT-3′
	Reverse primer: 5′-GACGACCGATTTGCACGTC-3′
IFN-α	Probe: 5′-(FAM)-CTCAACCGGATCCACCGCTACACC-(BHQ)-3′	Intronless	U07868	38
	Forward primer: 5′-GACAGCCAACGCCAAAGC-3′
	Reverse primer: 5′-GTCGCTGCTGTCCAAGCATT-3′
IFN-β	Probe: 5′-(FAM)-TTAGCAGCCCACACACTCCAAAACACTG-(BHQ)-3′	Intronless	X92479	38
	Forward primer: 5′-CCTCCAACACCTCTTCAACATG-3′
	Reverse primer: 5′-TGGCGTGCGGTCAAT-3′
IFN-γ	Probe: 5′-(FAM)-TGGCCAAGCTCCCGATGAACGA-(BHQ)-3′	3/4	Y07922	19
	Forward primer: 5′-GTGAAGAAGGTGAAAGATATCATGGA-3′
	Reverse primer: 5′-GCTTTGCGCTGGATTCTCA-3′
IL-4	Probe: 5′-(FAM)-AGCAGCACCTCCCTCAAGGCACC-(BHQ)-3′	3/4	AJ621735	41
	Forward primer: 5′-AACATGCGTCAGCTCCTGAAT-3′
	Reverse primer: 5′-TCTGCTAGGAACTTCTCCATTGAA-3′
IL-6	Probe: 5′-(FAM)-AGGAGAAATGCCTGACGAAGCTCTCCA-(BHQ)-3′	3/4	AJ250838	19
	Forward primer: 5′-GCTCGCCGGCTTCGA-3′
	Reverse primer: 5′-GGTAGGTCTGAAAGGCGAACAG-3′
IL-8	Probe: 5′-(FAM)-TCTTTACCAGCGTCCTACCTTGCGACA-(BHQ)-3′	1/2	AJ0099800	59
	Forward primer: 5′-GCCCTCCTCCTGGTTTCAG-3′
	Reverse primer: 5′-TGGCACCGCAGCTCATT-3′
IL-15	Probe: 5′-(FAM)-AAGTTGCAAATCTTGCATTTCCATTTTTCCA-(BHQ)-3′	4/5	AJ416937	19
	Forward primer: 5′-TAGGAAGCATGATGTACGGAACAT-3′
	Reverse primer: 5′-TTTTTGCTGTTGTGGAATTCAACT-3′
IL-18	Probe: 5′-(FAM)-CCGCGCCTTCAGCAGGGATG-(BHQ)-3′	4/5	AJ276026	19
	Forward primer: 5′-AGGTGAAATCTGGCAGTGGAAT-3′
	Reverse primer: 5′-ACCTGGACGCTGAATGCAA-3′

^aFAM, 6-carboxyfluorescein; BHQ, Black Hole Quencher dye (Biosearch Technologies).