TABLE 1.
Selected proteins in an IP of UL44 from HCMV-infected cells
| Banda | Protein identified | GenBank no. | Species | No. of peptidesb | % Coveragec |
|---|---|---|---|---|---|
| 1 | HCMV major capsid protein | P16729 | 16 | 12.9 | |
| Dead box protein 9 | Q08211 | Human | 3 | 2.1 | |
| 2 | HCMV ssDNA-binding protein UL57 | P17147 | 7 | 6.3 | |
| AP-3 complex subunit 1 | O00203 | Human | 6 | 5.7 | |
| Regulator nonsense transcript 1 | Q92900 | Human | 1 | 0.9 | |
| Pre-mRNA processing factor | O75400 | Human | 1 | 0.9 | |
| 3 | HCMV tegument protein US22 | P09722 | 8 | 11.8 | |
| HCMV replication factor UL84 | P16727 | 5 | 8.0 | ||
| Stress-70 protein | P38646 | Human | 3 | 5.4 | |
| Lamin-A/C | P02545 | Human | 1 | 2.6 | |
| 4 | HCMV tegument protein pp65 | P06725 | 35 | 58.6 | |
| HCMV Pol accessory subunit UL44 | P16790 | 8 | 19.9 | ||
| Ig gamma chain | P01867 | Murine | 5 | 18.6 | |
| Ig alpha chain | P01878 | Murine | 4 | 16.0 | |
| Heterogeneous nuclear ribonucleoprotein K | P61978 | Human | 3 | 9.1 | |
| Ub cross-reactive protein | P05161 | Human | 2 | 13.4 | |
| HCMV alkaline nuclease UL98 | P16789 | 2 | 2.9 | ||
| 5 | HCMV Pol accessory subunit UL44 | P16790 | 60 | 93.8 |
a
The bands indicated by the arrows in Fig. 1 were analyzed by liquid chromatography-tandem mass spectrometry.
b
Number of peptides from each protein identified.
c
Percentage of the total protein sequence that is represented by the peptides identified.