TABLE 2.
Yeast two-hybrid assaysa
| DNA-binding domain fusion protein | Activation domain fusion protein | β-Gal expressionb |
|---|---|---|
| Gal4BD-UL84 | − | |
| Gal4AD-UL44 | − | |
| Gal4BD-UL84 | Gal4AD-UL44 | + |
| Gal4AD-UL44P85G | − | |
| Gal4BD-UL84 | Gal4AD-UL44P85G | + |
| Gal4AD-UL44L86A/L87A | − | |
| Gal4BD-UL84 | Gal4AD-UL44L86A/L87A | + |
| Gal4AD-UL44F121A | − | |
| Gal4BD-UL84 | Gal4AD-UL44F121A | + |
| Gal4AD-UL44I135A | − | |
| Gal4BD-UL84 | Gal4AD-UL44I135A | + |
| Gal4BD-UL54 | − | |
| Gal4BD-UL54 | Gal4AD-UL44 | + |
| Gal4BD-UL54 | Gal4AD-UL44P85G | + |
| Gal4BD-UL54 | Gal4AD-UL44L86A/L87A | + |
| Gal4BD-UL54 | Gal4AD-UL44F121A | + |
| Gal4BD-UL54 | Gal4AD-UL44I135A | − |
a
UL84 or UL54 was fused to Gal4BD, and wild-type or mutant UL44 proteins were fused to Gal4AD and assayed for interaction by β-Gal filter assays.
b
+, blue color detected after 2 to 3 h of incubation; −, no signal detected after 24 h of incubation.