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Results from NMR experiments. The differences in the HSQC (A) and Cα (B) chemical shifts between WT and D187N are displayed. The {1H-15N} NOEs are displayed as (Isat − Iref)/Iref for WT (C) and D187N (D). The more negative values are indicative of motion on the nanosecond time scale. The C-terminal tail of D187N begins falling off at Ala-252 compared with Glu-258 in WT, suggesting that six more residues are unstructured in the mutant.
