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. 1983 Sep;18(3):658–662. doi: 10.1128/jcm.18.3.658-662.1983

Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage.

C L Howell, M J Miller
PMCID: PMC270870  PMID: 6313747

Abstract

Cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 M SP) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees C over time. Recovery from 0.2 M SP was uniformly better. More tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 M SP virus stocks as compared with viruses stored in 70% sorbitol. Although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing diluted virus suspensions generally resulted in diminished recovery. Similar stabilizing effects on respiratory syncytial virus and herpes simplex virus type 1 infectivities were observed when stocks were preserved in 0.2 M SP, as compared with 70% sorbitol. Overall, 0.2 M SP was better than 70% sorbitol for stabilizing cytomegalovirus, varicella-zoster virus, respiratory syncytial virus, and herpes simplex virus type 1 infectivities under the conditions tested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN E. G., KANEDA B., GIRARDI A. J., SCOTT T. F. M., SIGEL M. M. Preservation of viruses of the psittacosis-lymphogranuloma venerum group and herpes simplex under various conditions of storage. J Bacteriol. 1952 Mar;63(3):369–376. doi: 10.1128/jb.63.3.369-376.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ash R. J., Barnhart E. R. Optimal cooling and warming rates in the preservation of herpes simplex virus (type 2). J Clin Microbiol. 1975 Sep;2(3):270–271. doi: 10.1128/jcm.2.3.270-271.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brunell P. A. Separation of infectious Varicella-Zoster virus from human embryonic lung fibroblasts. Virology. 1967 Apr;31(4):732–734. doi: 10.1016/0042-6822(67)90207-3. [DOI] [PubMed] [Google Scholar]
  5. Darlington R. W., Moss L. H., 3rd The envelope of Herpesvirus. Prog Med Virol. 1969;11:16–45. [PubMed] [Google Scholar]
  6. Farrant J., Morris G. J. Thermal shock and dilution shock as the causes of freezing injury. Cryobiology. 1973 Jun;10(2):134–140. doi: 10.1016/0011-2240(73)90019-9. [DOI] [PubMed] [Google Scholar]
  7. Feldman R. A. Cytomegaloviruses in stored urine specimens. A quantitative study. J Pediatr. 1968 Oct;73(4):611–614. doi: 10.1016/s0022-3476(68)80280-x. [DOI] [PubMed] [Google Scholar]
  8. Grose C., Friedrichs W. E., Smith K. O. Cryopreservation of varicella-zoster virions without loss of structural integrity or infectivity. Intervirology. 1981;15(3):154–160. doi: 10.1159/000149225. [DOI] [PubMed] [Google Scholar]
  9. HAMBLING M. H. SURVIVAL OF THE RESPIRATORY SYNCYTIAL VIRUS DURING STORAGE UNDER VARIOUS CONDITIONS. Br J Exp Pathol. 1964 Dec;45:647–655. [PMC free article] [PubMed] [Google Scholar]
  10. Hondo R., Shibuta H., Matumoto M. An improved plaque assay for varicella virus. Arch Virol. 1976;51(4):355–359. doi: 10.1007/BF01317939. [DOI] [PubMed] [Google Scholar]
  11. Larew M. S., Myers M. G. Recovery of cytomegalovirus and Chlamydia trachomatis from vaginal tampons. J Med Virol. 1982;9(1):37–42. doi: 10.1002/jmv.1890090106. [DOI] [PubMed] [Google Scholar]
  12. Law T. J., Hull R. N. The stabilizing effect of sucrose upon respiratory syncytial virus infectivity. Proc Soc Exp Biol Med. 1968 Jun;128(2):515–518. doi: 10.3181/00379727-128-33054. [DOI] [PubMed] [Google Scholar]
  13. MAZUR P. KINETICS OF WATER LOSS FROM CELLS AT SUBZERO TEMPERATURES AND THE LIKELIHOOD OF INTRACELLULAR FREEZING. J Gen Physiol. 1963 Nov;47:347–369. doi: 10.1085/jgp.47.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MEDEARIS D. N., Jr OBSERVATIONS CONCERNING HUMAN CYTOMEGALOVIRUS INFECTION AND DISEASE. Bull Johns Hopkins Hosp. 1964 Mar;114:181–211. [PubMed] [Google Scholar]
  15. MERYMAN H. T. Preservation of living cells. Fed Proc. 1963 Jan-Feb;22:81–89. [PubMed] [Google Scholar]
  16. MUNK K., ACKERMANN W. W. Some properties of herpes simplex virus. J Immunol. 1953 Dec;71(6):426–430. [PubMed] [Google Scholar]
  17. Meurisse E. V. Laboratory studies on the varicella-zoster virus. J Med Microbiol. 1969 Aug;2(3):317–325. doi: 10.1099/00222615-2-3-317. [DOI] [PubMed] [Google Scholar]
  18. RAPP F., BENYESH-MELNICK M. Plaque assay for measurement of cells infected with zoster virus. Science. 1963 Aug 2;141(3579):433–434. doi: 10.1126/science.141.3579.433. [DOI] [PubMed] [Google Scholar]
  19. ROSANOFF E. I., HEGARTY C. P. PRESERVATION OF TISSUE CULTURED VARICELLA VIRUS IN THE FROZEN STATE. Virology. 1964 Feb;22:284–284. doi: 10.1016/0042-6822(64)90014-5. [DOI] [PubMed] [Google Scholar]
  20. SMITH K. O. RELATIONSHIP BETWEEN THE ENVELOPE AND THE INFECTIVITY OF HERPES SIMPLEX VIRUS. Proc Soc Exp Biol Med. 1964 Mar;115:814–816. doi: 10.3181/00379727-115-29045. [DOI] [PubMed] [Google Scholar]
  21. SPECK R. S., JAWETZ E., COLEMAN V. R. Studies on herpes simplex virus. I. The stability and preservation of egg-adapted herpes simplex virus. J Bacteriol. 1951 Mar;61(3):253–258. doi: 10.1128/jb.61.3.253-258.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schmidt N. J., Lennette E. H. Improved yields of cell-free varicella-zoster virus. Infect Immun. 1976 Sep;14(3):709–715. doi: 10.1128/iai.14.3.709-715.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith T. F., Weed L. A., Pettersen G. R., Segura J. W. Recovery of Chlamydia and Genital Mycoplasma transported in sucrose phosphate buffer and urease color test medium. Health Lab Sci. 1977 Jan;14(1):30–34. [PubMed] [Google Scholar]
  24. Vonka V., Benyesh-Melnick M. Interactions of human cytomegalovirus with human fibroblasts. J Bacteriol. 1966 Jan;91(1):213–220. doi: 10.1128/jb.91.1.213-220.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WELLER T. H., HANSHAW J. B. Virologic and clinical observations on cytomegalic inclusion disease. N Engl J Med. 1962 Jun 14;266:1233–1244. doi: 10.1056/NEJM196206142662401. [DOI] [PubMed] [Google Scholar]
  26. WELLER T. H., WITTON H. M., BELL E. J. The etiologic agents of varicella and herpes zoster; isolation, propagation, and cultural characteristics in vitro. J Exp Med. 1958 Dec 1;108(6):843–868. doi: 10.1084/jem.108.6.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wallis C., Melnick J. L. Stabilization of enveloped viruses by dimethyl sulfoxide. J Virol. 1968 Sep;2(9):953–954. doi: 10.1128/jvi.2.9.953-954.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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