Skip to main content
. 2009 Jun 18;106(27):11131–11136. doi: 10.1073/pnas.0812789106

Fig. 3.

Fig. 3.

MAPK signaling is activated by the CB2 selective agonist JWH133 in spermatogonia. (A) Dose-dependent activation of ERK phosphorylation by JWH133 in mouse spermatogonia. Isolated mouse spermatogonia were incubated with or without 10−8–5 × 10−6 M JWH133 for 15 min at 37 °C. At the end of the incubation, cell extracts were prepared and the immunoblot was performed as described in Methods. (B) Time-course analysis of JWH133-stimulated phosphorylation of ERKs in spermatogonia. Isolated spermatogonia were incubated with 10−6 M JWH133, for 0–60 min at 37 °C. At the end of the incubation, cell extracts were prepared for Western blot analysis with anti-phosphoERKs antibody. Densitometric analysis of phosphoERKs is shown on Right. (C) Isolated spermatogonia were treated with the CB2-selective agonist JWH133, or with the CB1-selecive agonist ACEA, or with c-Kit ligand (KL), as positive control, and pERKs activation was evaluated. Densitometric analysis is shown in Right. (D) The effects of preincubation (15 min) with the CB2-selective antagonist AM630 (1 μM) on the phosphorylation of ERKs by JWH133 (1 μM) in mouse spermatogonia was tested at different times (5 min to 24 h).