Figure 1.

Histological analysis of a subcutaneous mouse model of biomaterial implantation was performed by analysis of tissue sections of the implant and lesion area at 2, 4, and 10 weeks postimplantation. A–D: H&E-stained sections of the implant and lesion area at 4 weeks. E: Immunohistochemical staining for Ly-6C showing neutrophil infiltration around a section of implant. F: Adhesion cell density. G: Percentage of macrophage fusion. H: The extent of fibrosis was quantified by computer-assisted image analysis as previously reported. Positive blue stain for collagen was selected based on its color ranges and the proportional area in each image was quantified and expressed as a percentage of the image. The statistical significance of week 2 was compared with the control group using Student’s t-test. Week 4 and 10 were compared with control and 2 weeks using analysis of variance. There was no significant difference between week 4 and 10 when analyzed by Student’s t-test. The data represent the mean ± SEM. I–K: Masson’s trichrome stained sections of implant and lesion area at 2, 4, and 10 weeks postimplantation shows areas of fibrosis. Blue stain is for collagen, red/pink is for cellular cytoplasm, and black is for nuclei. Arrows indicate areas of fibrosis. *P < 0.05.