Table 1.
Antibodies Used for Immunohistochemistry Studies
| Antibody | Company | Dilution | 2° Antibody |
|---|---|---|---|
| F4/80 | Invitrogen (Carlsbad, CA) | 1/25 | Goat anti-rat |
| CD11c | BD PharMingen (San Diego, CA) | 1/100 | Goat anti-rat |
| CD11b | Santa Cruz Biotechnologies, Inc. (Santa Cruz, CA) | 1/100 | Rabbit anti-goat |
| Ly-6C | BD PharMingen | 1/75 | Goat anti-rat |
| B220 | BD PharMingen | 1/200 | Goat anti-rat |
| CD3 | DAKO (Dako, Denmark) | 1/100 | Goat anti-rabbit |
| IL-4 | Santa Cruz Biotechnologies, Inc. | 1/50 | Rabbit anti-goat |
| IL-13 | Santa Cruz Biotechnologies, Inc. | 1/75 | Rabbit anti-goat |
| IL-10 | Santa Cruz Biotechnologies, Inc. | 1/50 | Rabbit anti-goat |
| IFN-γ | Invitrogen | 1/50 | Rabbit anti-goat |
| TNF-α | Invitrogen | 1/100 | Rabbit anti-goat |
| IL-6 | R&D Systems (Minneapolis, MN) | 1/300 | Rabbit anti-goat |
| MCP-1 | Santa Cruz Biotechnologies, Inc. | 1/50 | Rabbit anti-goat |
| TGF-β1/2/3 | Santa Cruz Biotechnologies, Inc. | 1/100 | Goat anti-rabbit |
Antibody dilutions were determined for optimal staining intensity.