Table 1.
Proteolytic Activity in Fecal Supernatants from Healthy Subjects (n = 7) and UC Patients (n = 12)
| Healthy | UC | P value | |
|---|---|---|---|
| Proteolytic activity (U/mg protein) | 73.10 ± 6.12 | 127.50 ± 27.51 | P < 0.05 |
| Serine protease activity among total proteases (inhibition with AEBSF) | |||
| U/mg protein | 23.25 | 57.89 | P < 0.05 |
| Percentage | 31.8% | 45.4% | P < 0.05 |
| Cathepsin G activity among serine proteases (inhibition with SCGI) | |||
| U/mg protein | 1.62 | 8.68 | P < 0.05 |
| Percentage | 7% | 15% | P < 0.05 |
Serine proteases activity among total proteolytic activity of fecal supernatants from healthy subjects and UC patients, was calculated after incubation of fecal supernatants with wide range serine protease inhibitor AEBSF (4-(2-aminoethyl)benzenesulphonyl fluoride hydrochloride). Cathepsin G activity among serine proteases in fecal supernatants from healthy subjects and UC patients, was calculated after incubation with specific Cathepsin G inhibitor (SCGI).