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. 2009 Jul;175(1):235–247. doi: 10.2353/ajpath.2009.080967

Figure 5.

Figure 5

Postnatal overexpression of Galgt2 inhibits the development of muscle pathology in Sgca−/− mice. A: Sgca−/− muscles infected with AAV1-CMV-Galgt2 show a range of increased Galgt2 mRNA expression, as measured by QRT-PCR, in four separate experiments. TA, tibialis anterior; Gas, gastrocnemius. B: Infected muscles overexpressing Galgt2 were visualized by immunostaining with CT2, an antibody to the CT carbohydrate (green). Central nuclei were evident on counterstaining with an antibody to β dystroglycan (red). Scale bar =100 μm. C: Myofiber diameters were measured in Galgt2-overexpressing myofibers and compared with muscles not overexpressing transgene. D: Muscles overexpressing Galgt2 had significantly fewer myofibers with central nuclei than muscles not overexpressing transgene (P < 0.001 for comparison in Gastroc and TA). Errors are SD for n = 3 to 4 animals per condition in C and D. Errors in (A) are SEM for n = 9 measurements per condition.