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. 2009 Jul;175(1):342–354. doi: 10.2353/ajpath.2009.080931

Figure 4.

Figure 4

Depletion of NOLC1 suppressed MDM2 expression in NPC-TW03 cells. A: Knockdown of NOLC1 expression suppressed the MDM2 mRNA expression in NPC-TW03 cells. Total RNAs derived from NPC-TW03 and shNOLC1-1-NPC-TW03 cells were analyzed by reverse transcription-PCR. Both NOLC1 and MDM2 mRNA expression were suppressed. The β-actin cDNA was used as an internal control. B: Western blot analysis of NOLC1 and MDM2 protein expression in NPC-TW03 and shNOLC1–1-NPC-TW03 cells. α-tubulin was used as the internal control in each lane. C: QRT-PCR analysis of the expressions of the NOLC1 (top) and MDM2 (bottom) genes in NPC-TW03 and shNOLC1-1-NPC-TW03 xenograft tumors. The mRNA of NOLC1 and MDM2 expression in the shNOLC1-NPC xenograft was also markedly suppressed. The data were obtained from triplicate experiments and normalized to the β-actin cDNA as the control. **P < 0.01.