Table 1.
The Primers Used for RT-PCR and QRT-PCR
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A. For RT-PCR
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|---|---|---|---|
| UniGene number | Gene symbol | Official full name | Primers |
| Hs.467020 | BCL-2 | B-cell CLL/lymphoma 2 | 5′-ACTTGTGGCCCAGATAGGCACCCAG-3′ |
| 5′-CGACTTCGCCGAGATGTCCAGCCAG-3′ | |||
| Hs.631546 | BAX | BCL2-associated X protein | 5′-GCTCTGAGCAGATCATGAAGACAG-3′ |
| 5′-CACAAAGATGGTCACGGTCTGC-3′ | |||
| Hs.2490 | CASP1 | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) | 5′-TTTGATTGACTCCGTTATTC-3′ |
| 5′-TCTCTGCCGACTTTTGTTTC-3′ | |||
| Hs.23960 | CCNB1 | cyclin B1 | 5′-GAAGCTACTGGAAACATG-3′ |
| 5′-CTACAGCTCGTTGTATGA-3′ | |||
| Hs.153752 | CDC25B | cell division cycle 25B | 5′-CACGCCCGTGCAGAATAAGC-3′ |
| 5′-ATGACTCTCTTGTCCAGGCTACAGG-3′ | |||
| Hs.370771 | CDKN1A | cyclin-dependent kinase inhibitor 1A (p21, Cip1) | 5′-ATGTCAGAACCGGCTGGGGATG-3′ |
| 5′-GCAGGCCAAGGCCCCGCAC-3′ | |||
| Hs.644056 | CSNK2A1 | casein kinase 2, alpha 1 | 5′-ATGACCACCAGTCACGGCTTAC-3′ |
| 5′-GGTTCAGACACGGTGCTTCTG-3′ | |||
| Hs.488293 | EGFR | epidermal growth factor receptor | 5′-CATAGACGACACCTTCCTCC-3′ |
| 5′-GGGTCTAAGAGCTAATGCGG-3′ | |||
| Hs.244139 | FAS | Fas (TNF receptor superfamily, member 6) | 5′-TAGCTCCTATATTTTCGGCTT-3′ |
| 5′-CTCACCAGCAACACCAAGTGC-3′ | |||
| Hs.396530 | HGF | hepatocyte growth factor | 5′-ACTGGCTCTTTTAGGCACTGACTC-3′ |
| 5′-TGTTCCCTTGTAGCTGCGTCCTTT-3′ | |||
| Hs.132966 | MET | Met proto-oncogene (hepatocyte growth factor receptor) | 5′-ACTCCCCCTGAAAACCAAAGCC-3′ |
| 5′-GGCTTACACTTCGGGCACTTAC-3′ | |||
| Hs.567303 | MDM2 | Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) | 5′-GCAGGGGAGAGTGATACAGAT-3′ |
| 5′-GATGGCTGAGAATAGTCTTCA-3′ | |||
| Hs.513617 | MMP2 | Matrix metallopeptidase 2 | 5′-GGGGCCTCTCCTGACATT-3′ |
| 5′-CATTCCCTGCAAAGAACACA-3′ | |||
| Hs.297413 | MMP9 | Matrix metallopeptidase 9 | 5′-TGGGCTACGTGACCTATGAC-3′ |
| 5′-CAAAGGTGAGAAGAGAGGGC-3′ | |||
| Hs.202453 | MYC | v-myc myelocytomatosis viral oncogene homolog | 5′-GTGGCACCTCTTGAGGACCA-3′ |
| 5′-TGGTGCTCCATGAGGAGACA-3′ | |||
| Hs.463456 | NME1 | Non-metastatic cells 1, protein (NM23A) | 5′-TGCTGCGAACCACGTGGGT-3′ |
| 5′-ATGTGGTCTGCCCTCCTGT-3′ | |||
| Hs.645227 | TGFB1 | Transforming growth factor, beta 1 | 5′-CTCCGAGAAGCGGTACCTGAAC-3′ |
| 5′-CACTTGCAGTGTGTTATCCCT-3′ | |||
| Hs.522632 | TIMP-1 | TIMP metallopeptidase inhibitor 1 | 5′-CTGGAAAACTGCAGGATGGA-3′ |
| 5′-CGCTGAGCTAAGGTCAGGCT-3′ | |||
| Hs.633514 | TIMP-2 | TIMP metallopeptidase inhibitor 2 | 5′-CTCATTGCAGGAAAGGCCGA-3′ |
| 5′-TGGGTGGTGCTCAGGGTGTC-3′ | |||
| Hs.591665 | TIMP-4 | TIMP metallopeptidase inhibitor 4 | 5′-CCAGAGGTCAGGTGGTAA-3′ |
| 5′-ACAGCCAGAAGCAGTATC-3′ | |||
| Hs.241570 | TNF | Tumor necrosis factor (TNF superfamily, member 2) | 5′-CTTCTGCCTGCTGCACTTTGGA-3′ |
| 5′-TCCCAAAGTAGACCTGCCCAGA-3′ | |||
| Hs.81791 | TNFRSF11B | Tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) | 5′-AGGAAATGCAACACACGACAAC-3′ |
| 5′-AGGAACAGCAAACCTGAAGAATG-3′ | |||
| Hs.156346 | TOP2A | topoisomerase (DNA) II alpha 170kDa | 5′-TGGTCAGAAGAGCATATGAT-3′ |
| 5′-CTCACAATCTGATCAGCTAC-3′ | |||
| Hs.408312 | TP53 | Tumor protein p53 | 5′-CTATGTCGAAAAGTGTTTCTGTCATC-3′ |
| 5′-CAGCCAAGTCTGTGACTTGCACGTAC-3′ | |||
| Hs.111779 | SPARC | Secreted protein, acidic, cysteine-rich (osteonectin) | 5′-ACTGAAGCTTCCCAGCACCATG-3′ |
| 5′-GAGAGGATCCGGTACTGTGG-3′ | |||
| Hs.585572 | SOX5 | SRY (sex determining region Y)-box 5 | 5′-CAACCTTGGTGCTGCTGTATCT-3′ |
| 5′-GTCTTGGGTTTAGCTGATAGGTTCA-3′ | |||
| Hs.73793 | VEGF | Vascular endothelial growth factor A | 5′-CGATCGTTCTGTATCAGTCTTTCC-3′ |
| 5′-GAAGTGGTGAAGTTCATGGATGTC-3′ | |||
| Hs.523238 | NOLC1 | Nucleolar and coiled-body phosphoprotein 1 | 5′-AGAAAAGAAAAAGGCGGCAG-3′ |
| 5′-TCCTCATCAAGACCCTCACC-3′ | |||
| Hs.520640 | ACTB | Actin, beta | 5′-CACTCTTCCAGCCTTCCTTC-3′ |
| 5′-GCCATGCCAATCTCATCTTG-3′ | |||
| Hs.544577 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 5′-CGGGAAGCTTGTGATCCATGG-3′ |
| 5′-GGCAGTGATGGCATGGACTG-3′ | |||
| (table continues) | |||