Table 2.
Antibody | Manufacturer | Dilution |
---|---|---|
Anti-factor H goat polyclonal | Calbiochem, Merck Bioscience | 1:50 |
Rabbit polyclonal to complement C3 | Abcam | 1:10 |
C3b/iC3b/C3c Rat monoclonal to mouse | Hycult biotechnology | 1:50 |
Rabbit polyclonal to C5 | Abcam | 1:10 |
Rabbit Polyclonal to C5b-9 (MAC) | Abcam | 1:100 |
Fluorescein conjugated Rat anti-mouse F4/80 | Invitrogen | 1:100 |
Alexa Fluor 594 conjugated to isolectin | Invitrogen | 1:50 |
Wherever possible individual animals were used in multiple experiments and eyes harvested bilaterally. All antibodies came from UK suppliers.