Figure 4.

ApoE antibodies stimulate HL displacement. HDL from three different subjects was pooled and incubated with an anti-apoE antibody (6C5, 1D7, or 3H1) for 2 hours in serum-free media. CHO-hHL cells were cultured in 6-well plates and treated with HDL ± anti-apoE antibody for 45 minutes. HL displacement from the cell surface was measured by immunochemical analysis and is shown relative to control HDL displacement, normalized to total cell protein. Values are the mean ± SD of triplicate determinations from three displacement experiments. Significant difference relative to control HDL, *P < 0.01.