FIGURE 10.

Induction of RpoE and CpxR/A pathways. Exponentially grown cultures of wild-type K-12 strain W3110 or its derivatives lacking waaC, lpxL, and lpxM alone and their deletion combinations with lpxP, carrying either rpoHP3-lacZ (A) or cpxP-lacZ fusion (B) on the chromosome were grown in M9 minimal medium under permissive growth conditions. Cultures were diluted to an A₆₀₀ of ∼0.02 and grown at 30 °C in the same medium. Aliquots of samples were drawn and analyzed for β-galactosidase activity. Error bars represent S.E. of four independent measurements. C–E, cultures of wild-type, ΔwaaC, and its derivatives were grown at 23 °C in M9 medium to logarithmic phase, harvested by centrifugation, and equivalent amounts of cells were used to prepare whole cell lysates. Proteins were resolved by 12% SDS-PAGE and transferred to nitrocellulose membranes. Blots were probed with antiserum raised against HtrA (C), RseB (D), and PpiD (E). The genotype of each strain used is indicated.