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. 2009 Apr 13;284(23):15416–15424. doi: 10.1074/jbc.M809384200

FIGURE 1.

FIGURE 1.

Tfg2p has a PY-NLS that is recognized by Kap104p. A, domain organization of Tfg2p. B, sequence of the PY-NLS. Residues mutated in Fig. 2 are underlined. C, ITC profile of MBP-Tfg2p PY-NLS injected into Kap104p. D, wild-type and kap104–16 temperature-sensitive cells expressing GFP-GST-Tfg2p PY-NLS were analyzed by fluorescence microscopy at permissive (room temperature) and non-permissive (37 °C) temperatures.