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Tfg2p has a strong epitope 3. A, binding assays of recombinant Kap104p with immobilized wild-type or mutant Tfg2p PY-NLS. Proteins were visualized by Coomassie staining. The asterisk labels a degradation product. B, cellular localization of GFP-GST-Tfg2 PY-NLS mutants in wild-type yeast cells analyzed by fluorescence microscopy and phase contrast. GFP-GST is included as a control.
