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. 2009 Apr 13;284(23):15416–15424. doi: 10.1074/jbc.M809384200

FIGURE 5.

FIGURE 5.

Other karyopherins bind Tfg2p, but they do not import it into the nucleus. A, binding assay of recombinant karyopherins with immobilized full-length Tfg2p. Proteins were visualized by Coomassie stain. B, binding assay of recombinant karyopherins with immobilized full-length Tfg2p in the presence and absence of RanGTP. Proteins were visualized by Coomassie stain. C, GFP-GST-Tfg2p fragments were expressed in wild-type yeast cells and analyzed by fluorescence microscopy and phase contrast. Only the PY-NLS (165–214) is nuclear.