FIGURE 2.

GA inhibition of Kir2.1 current in HEK293 cells. A, time course of ⁸⁶Rb⁺ efflux from cells treated with GA at indicated concentrations (μm) for 3 h. B, Kir2.1 currents evoked by voltage-clamp ramps from −120 mV to 0 mV from holding potential of −40 mV in nontreated cells and in cells treated with 50 nm or 500 nm GA for 3 h. Data represent mean ± S.E., n = 6–10. C, Kir2.1 currents evoked by voltage clamp ramps from −120 mV to 0 mV from holding potential of −40 mV before and after acute application of 10 μm GA. Data represent mean ± S.E., n = 3. D, dose-response relationship for acute and chronic GA inhibition. Inward current was measured at −117 mV. Data points represent mean ± S.E. The number of cells for each data point is indicated in parentheses.