FIGURE 5.

GA does not affect HERG and Kir1.1 channels. A, time course of ⁸⁶Rb⁺ efflux from HEK293 cells stably expressing HERG channel. Cells were incubated with GA at indicated concentrations (μm) for 2 h. ⁸⁶Rb⁺ efflux was induced by 50 mm KCl and repressed by 10 μm E-4031. Baseline efflux was measured at external KCl of 5 mm. B, relative amount of released ⁸⁶Rb⁺ in 3 min via HERG channel. Control cells were not treated with GA. Numbers indicate GA and E-4031 concentrations in μm. Bars represent mean ± S.E., n = 3–5. C, time course of ⁸⁶Rb⁺ efflux from HEK293 cells with Tet-inducible Kir1.1 channels. Cells were incubated with GA at indicated concentrations (μm) for 2 h. D, relative amount of released ⁸⁶Rb⁺ in 3 min via Kir1.1 channel. Numbers indicate GA concentrations in μm. Bars represent mean ± S.E., n = 3–5.