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Stability of acetylation mutants of NGN is affected by acetylation-blocking. Degradation assays in interphase egg extract were performed on NGN constructs, as indicated, in vitro translated either in the absence (Control) or in the presence of citrate synthase, oxaloacetate, and desulfo CoA (Acetyl block). Assays were analyzed by autoradiography (left panels) and quantitative phosphorimaging analysis (right graphs).
