Effect of nonspecific Ca2+ channel inhibition on aspects of Class II AMP-mediated apoptosis. Total ATP (A), caspase-3/7-like activity (B), TUNEL activity (C), and flow cytometry after staining with rhodamine 123 (D) were measured in cells with and without pretreatment with FFA. C-II AMP and FFA treatment was done according to conditions described above. BAF, FCCP, and nuclease treatment were done as described previously (5).