TABLE 3.
Values of the dissociation constantKd of the binding of NNI-1, NNI-3, and NNI-4 with NS5B570-Con1 in the absence and presence of HCV cIRES-378nt RNA
All of the values shown are mean and S.D. values calculated from three or more independent experiments. The experiments were carried out as described under “Experimental Procedures.”
| Compounds | HCV cIRES | Preincubation of NS5B and cIRES |
Kd |
|
|---|---|---|---|---|
| 10 mina | 1 h 10 mina | |||
| μm | ||||
| NNI-1 | − | − | 0.019 ± 0.010 | 0.029 ± 0.013 |
| + | 0 h | 0.019 ± 0.008 | 0.027 ± 0.012 | |
| + | 1 h | 0.018 ± 0.006 | 0.022 ± 0.009 | |
| NNI-3 | − | − | 0.026 ± 0.011 | 0.025 ± 0.005 |
| + | 0 h | 0.086 ± 0.012 | 0.099 ± 0.036 | |
| + | 1 h | 0.082 ± 0.020 | 0.105 ± 0.018 | |
| NNI-4 | − | − | 25 ± 2.4 | 26 ± 12 |
| + | 0 h | 123 ± 9.0 | 72 ± 1.8 | |
| + | 1 h | 162 ± 24 | 89 ± 0.2 | |
a Time of NNI incubation with NS5B or NS5B-cIRES.