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Surface dilution kinetics for PI-PLC tyrosine mutant enzymes. Data for Y247S/Y251S (●), Y246S/Y247S/Y248S (□), and Y246S/Y247S/Y248S/Y251S (▵) are shown. The PI bulk concentration was fixed at 6 mm with the diC7PC increasing from 18 to 60 mm; the mole fraction of PI, XPI, in these mixed micelle assays is shown on the top of the graph.
