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. 2009 Apr 7;284(23):15650–15658. doi: 10.1074/jbc.M900814200

FIGURE 1.

FIGURE 1.

SDS-PAGE and Western blot analysis of the recombinant FH19–20 mutant constructs. Samples of the proteins (0.3 μg) were resolved by SDS-PAGE gels in non-reducing Laemmli sample buffer. The proteins were visualized by Coomassie Blue staining (upper panel) and Western blotting (lower panel), where the proteins were detected with polyclonal goat anti-human FH antibody and horseradish peroxidase-conjugated donkey anti-goat antibody. The aHUS-associated mutations are in bold. Dashed lines indicate composition of separate lanes from the gels.