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Interaction domain mapping. A, left, schematic representation of GST-tagged KMT3a deletion mutants used for the interaction assay. Middle, silver stain of purified recombinant GST-KMT3a deletion mutants. Right, results from the nickel agarose pulldown assay using His-HnRNP-L. B, left, schematic representation of His-tagged HnRNP-L deletion mutants. Right, results from nickel pulldown assays using deletion mutants of His-tagged HnRNP-L and of GST-KMT3a, as indicated.
