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. 2009 Apr 6;284(23):15708–15716. doi: 10.1074/jbc.M900437200

FIGURE 3.

FIGURE 3.

Quantification of construct expression at the plasma membrane. Intensity ratio of donor to acceptor (D/A) for various combinations of constructs. GPI-CFP, GPI-mCFP, and GPI-YFP are raft markers; TMD-HA-YFP, TMD-HA-mYFP, TMD-HA-CFP, TMD-HA-mCFP, and TMD-HAC3S-YFP are different TMD-HA variants. m, A206K mutation preventing any potential of dimerization; C3S, mutation of cysteines 551, 559, and 562 to serine. SFV-E2-mCFP is the non-raft protein based on the Semliki Forest virus E2 spike protein (see supplemental “Experimental Procedures” and under “FLIM-FRET Experiments with the Transmembrane Domain SFV-E2-mCFP Fusion Protein.”). Intensities were measured at 25 °C. Data represent the mean ± S.E.