Toxicity of small molecule inhibitors in cell culture under conditions of tests of inhibition of processing
J774A.1 murine mac ro phages were incubated in RB* with DC1 (20 ×Ki), DC2 (20 ×Ki), DC3 (20 ×Ki), DC4 (20 ×Ki), or B3 (2 ×Ki) for times as shown. Toxicity was determined as described under “Anthrax Toxin Killing Assays” under “Experimental Procedures.” Cells in triplicate wells (∼600 cells per well) were counted for each time point. Average S.E.: 8.3% for DC1, 7.6% for DC2, 7.2% for DC3, 10.3% for DC4, and 13.9% for B3.