FIGURE 6.

Dbs increases FAK phosphorylation in a Cdc42- and Ack1-dependent manner. A, T47D cells that stably express vector (Vec), onco-Dbs, or proto-Dbs were cultured on collagen I. Lysates were prepared and examined by Western blot for levels of total Src, Src phosphorylated on Tyr-418 (p-Src), total FAK, and FAK phosphorylated on Tyr-397 (p-FAK). B, the cells were also transfected with siRNAs (si) targeting Cdc42 and Ack1 as indicated. Cell lysates were then collected and examined by Western blot for levels of total and phosphorylated (Tyr-397; p-FAK) FAK.