FIGURE 9.

Endogenous Dbs supports cell motility in MBA-MD-231 cells. A, cell lysates were collected from the indicated cell lines and examined by Western blot for expression of endogenous Dbs. Tubulin expression was also examined as a control for equivalent loading. B, MBA-MD-231 cells were cultured on collagen I and transfected with siRNAs (si) targeting Dbs at the indicated concentrations. Scrambled siRNAs (si Ctrl) were included as controls. Cell lysates were examined by Western blot for expression of Dbs as indicated. Lysates were also examined by affinity precipitation assays for levels of activated Cdc42 (Cdc42 GTP). C, MBA-MD-231 cells transfected with siRNAs for Dbs were also examined in transwell motility assays. Assays were performed on collagen I, and data were collected and presented as in Fig. 1.