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. 2009 Apr 14;284(23):15805–15814. doi: 10.1074/jbc.M901855200

FIGURE 1.

FIGURE 1.

Introduced cysteine mutations in TM2b and TM7 of the lateral gate of SecY. A, topology model of E. coli SecY based on a sequence alignment with the M. jannaschii SecY crystal structure (17). Highlighted are TM segments 2b and 7 and the amino acids residues selected for cysteine mutagenesis (red, Ser-87 and Phe-286; yellow, Met-83, Ile-86, and Phe-279). Also indicated is the OmpT cleavage site in cytoplasmic region C4. B, side view of the M. jannaschii SecY crystal structure showing the putative gate region (Protein Data Bank code 1RHZ (17)). Indicated are amino acids Ser-87 and Phe-286 in TM2b and TM7, respectively. The figure was created using MOLMOL (38) and POV-Ray.