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. 2009 Mar 25;284(23):15835–15846. doi: 10.1074/jbc.M807846200

FIGURE 2.

FIGURE 2.

Kinetic study of hSMUG1 cleavage of 5-substituted uracil analogues paired with guanine illustrating the gel electrophoretic assay (left) and time-dependent product ratios (right). Single-turnover reactions were performed at 37 °C with 50 nm substrate and 200 nm hSMUG1 in the reaction buffer, as described under “Materials and Methods.” Top, HmU:G as a substrate; bottom, CaU:G as a substrate.